林产化学与工业2007，Vol.27Issue(3)：43-48,6.
黑荆树树皮原花色素生物活性的研究
Investigation on Biological Activities of Proanthocyanidins from Black Wattle Bark
刘夏睿 1王飞1
作者信息
- 1. 南京林业大学,化学工程学院,江苏,南京,210037
- 折叠
摘要
Abstract
The extraction conditions of proanthocyanidins from bark of black wattle (Acacia mearnsii De Wilde) and their biological activities were investigated in this study. Different types of solvents such as water, methanol and ethanol were used as extraction solvent, respectively. Experiments were conducted to study the effects of temperature, liquid-solid ratio and concentration of ethanol on the extraction efficiency. The focus of this study is to evaluate the biological activities of the extracted proanthocyanidins products. The anti-tumor activity of proanthocyanidins was determined by MTT colorimetric method, and DPPH method referred to the radical scavenging capacity was used to test the anti-oxidation ability of the proanthocyanidins. The results showed that black wattle bark is rich in proanthocyanidins. Ethanol was considered to be a suitable solvent, in which there was higher extract yield and stronger biological activities of proanthocyanidins. It was found that the optimum conditions of extraction were as follows: concentration of ethanol 70%, temperature 60℃,and the ratio of solution to solid 7∶1. Furthermore, the results of anti-tumor test of proanthocyanidins crude products on human cancer cells cultured in vitro showed that the proanthocyanidins had medium effect on promyelocytic cell line HL-60, weak effect on human stomach adenocarcinoma BGC-823 and human hepatocellular carcianoma BEL-7402. Meanwhile, the results of radical scavenging ability assay indicated that the proanthocyanidins had strong anti-oxidation activity.关键词
黑荆树树皮/原花色素/抗肿瘤/清除自由基Key words
black wattle bark/proanthocyanidins/anti-tumor/radical scavenging分类
化学化工引用本文复制引用
刘夏睿,王飞..黑荆树树皮原花色素生物活性的研究[J].林产化学与工业,2007,27(3):43-48,6.
