昆虫学报2008，Vol.51Issue(4)：437-442,6.
野桑蚕酚氧化酶原基因cDNA的分子克隆及其特征
Molecular cloning and characterization of a cDNA encoding the prophenoloxidase gene in Bombyx mandarina
摘要
Abstract
Phenoloxidase plays an important role in defense mechanisms in insect immunity.The complemental deoxyribonucleic acid(cDNA)of Bombyx mandarins prophenoloxidase(PPO)gene was cloned by means of reverse transcription polymerase chain reaction(RT-PCR)and rapid amplification of cDNA ends(RACE).The cDNA was 2 134 bp in length and contained an open reading frame(ORF)of 2 082 bp which eneoded 693 amino acid residues.The deduced amino acid sequence showed a high identity to the reported sequence of PPO2 from other Lepidopterous insects and shared the typical structural features of PPO from other inseets.The results of RT-PCR showed that PPO mRNA was expressed in the integument,haemocyte,head,testis,ovary,fat body and midgut of 5th instar larvae and at different developmental stages of B.mandarina.These results provide molecular basis for further studying the function of ppo gene in B.mandarina.关键词
野桑蚕/酚氧化酶原基因/克隆/序列分析/组织表达Key words
Bombyx mandarina/prophenoloxidase gene/cloning/sequence analysis/tissue expression分类
生物科学引用本文复制引用
张永亮,黄重,李志清,张春冬,朱勇..野桑蚕酚氧化酶原基因cDNA的分子克隆及其特征[J].昆虫学报,2008,51(4):437-442,6.基金项目
教育部博士学科点专项基金(20060635008) （20060635008）
重庆市教委基金项目(040208) （040208）
