中德临床肿瘤学杂志（英文版）2006，Vol.5Issue(6)：438-441,4.
NF-κB诱骗剂——环状哑铃寡核苷酸抑制膀胱肿瘤细胞侵袭能力的研究
Experimental study on the invasiveness inhibition of bladder cancer cells by nuclear factor-κB decoy——circular dumbbell oligodeoxynucleotides
摘要
Abstract
Objective: To determine whether NF-κB is constitutively activated in human bladder cancer cell and, if so, to determine the invasiveness inhibition of bladder cancer cells by nuclear factor-κB decoy-circular dumbbell oligodeoxynucleotides (CD-ODN). Methods: NF-κBp65 activation was determined by immunohistochemical analysis of formalin-fixed, paraffin-embedded specimens from 38 cases of bladder transitional cell carcinoma patients. We quantified nuclear staining of RelA as a marker of NF-κBp65 activation. CD-ODN were transfected into human bladder cancer cell line BIU87 by lipofectamine. Luciferase reporter were applied to detecting NF-κB DNA binding activity. The expression levels of uPA were detected by RT-PCR and the cells' invasion ability by transwell cell culture chamber. Results: P65 excessive activation existed in tumor cell (P＜0.01), the activation degree correlated significantly with the expression of uPA (r=0.89, P＜0.01), as well as related to tumor invasion-related clinicopathological features such as lymphatic metastasis (P＜0.01) and pathological ranking (P＜0.05); After transfection with CD-ODN, the activation of NF-κB in BIU87 cell line was suppressed remarkably, the expression level of uPA was decreased and the cells' invasiveness was weakened as well. Conclusion: Excessively activated NF-κB is related to tumor progression possibly due to its transcriptional regulation of invasion-related factors such as uPA. CD-ODN can efficiently suppress DNA binding activity of NF-κB to reduce the invasive potency of tumor.关键词
decoy strategy/nuclear factor kappa B/bladder cancerKey words
decoy strategy/nuclear factor kappa B/bladder cancer分类
医药卫生引用本文复制引用
文博,周四维,杨为民..NF-κB诱骗剂——环状哑铃寡核苷酸抑制膀胱肿瘤细胞侵袭能力的研究[J].中德临床肿瘤学杂志（英文版）,2006,5(6):438-441,4.基金项目
Supported by a grant from the National Natural Science Foundation of China (No. 30271300). （No. 30271300）
