中国临床康复2004，Vol.8Issue(21)：4397-4399,3.
Caspase-3抑制剂对新生鼠缺氧缺血性脑损伤的保护作用
Protective effect of Caspase-3 inhibitor in neonatal rats with hypoxia-ischemia brain damage
张雨平 1赵聪敏 1奚敏 1王丽雁 1温恩懿 1于若谷1
作者信息
- 1. 解放军第三军医大学新桥医院儿科,重庆市,400037
- 折叠
摘要
Abstract
AIM:To observe the therapeutic effectiveness of acetyl-Asp-Glu-Val-Asp-ald-ehyde (AC-DEVD-CHO), a specific Caspase-3 inhibitor, in the treatment of hypoxia-ischemia brain-damaged(HIBD) neonatal rat models.METHODS:Different dose of AC-DEVD-CHO was administrated through cerebral ventricular injection in the neonatal rats after the establishment of HIBD model.The activities of Caspase-3 in the brains were measured after 24 hours by four-peptide-fluorescence-substance detection to determine the optimal dose.The therapeutic effectiveness of AC-DEVD-CHO was observed by means of histopathology and in situ apoptosis test, etc.RESULTS:The activity of Caspase-3 descended with the increasing dose of AC-DEVD-CHO.The difference was not significant when the dose reached 300 to 400 ng.The percentage of apoptotic neurocytes(30.2 ± 11.2%) in the cerebral cortex and hippocampus of HIBD neonatal rats in AC-DEVD-CHO 300 ng group was significantly lower than that(44.6 ±12.4%) of normal saline group( P ＜ 0.01).CONCLUSION:Caspase-3 inhibitor has cerebral protective effect in HIBD neonatal rats.关键词
脑缺血/脑缺氧/Caspase类分类
医药卫生引用本文复制引用
张雨平,赵聪敏,奚敏,王丽雁,温恩懿,于若谷..Caspase-3抑制剂对新生鼠缺氧缺血性脑损伤的保护作用[J].中国临床康复,2004,8(21):4397-4399,3.
