植物研究2005，Vol.25Issue(4)：441-443,3.
超声破碎拟南芥原生质体后的再生培养初报
The study on secondary culturation after the protoplasts of Arabidopsis thaliana broken by ultrasonic
摘要
Abstract
After the protoplasts of Arabidopsis thaliana was broken by the ultrasonic and filtrated by the filter membrane, we subcultured the organs such as chloroplast and nucleus. It' s observed that some chloroplasts were no fluorescence under the blue excitated light. The result indicated the ultrasonic had certain functions of destroying the chloroplasts. After being cultured for 115 h, the chloroplasts began to gather; and the nuclei obviously increased; also the bright green dots of endoplasmic reticulum appeared. As we all known the nuclei and endoplasmic reticulum are essential cell organs which form the new protoplast, so culturing cell organs may offer us some enlightenments about organs recombination and even for the cells reconstruction.关键词
拟南芥/原生质体/细胞器重组/荧光探针Key words
Arabidopsis thaliana/protoplast/organ recombination/fluorescent probe分类
农业科技引用本文复制引用
祖元刚,孟庆焕,郭晓瑞,孙佳音,刘红梅..超声破碎拟南芥原生质体后的再生培养初报[J].植物研究,2005,25(4):441-443,3.基金项目
Supported by the State Key Basic Research Project (G19990160) and the Key Project of Chinese Ministry of Education (104191) （G19990160）
