中山大学学报（医学科学版）2009，Vol.30Issue(4)：441-445,5.
骨肉瘤组织中MICA基因mRNA和蛋白的表达
Expression of MICA mRNA and Protein in Osteosarcoma
摘要
Abstract
[Objective] To investigate the expression of MICA mRNA and protein in osteosarcoma and give a more comprehensive understanding to its immune evasion.[Methods] RT-PCR was used to analyze MICA mRNA in 11 osteosarcoma tissues and 5 osteosarcoma cell lines.MICA expression was examined in 66 paraffin-embedded osteosarcoma tissues and 6 paraffin-embedded normal bone tissues by immunohistochemistry,and MICA protein in 9 fresh osteosarcoma tissues was detected by Western blot too.MICA surface expression was estimated by flow cytometry.[Results] Nine of eleven (81.8%) osteosarcoma specimens and all of the five cell lines consistently expressed MICA mRNA.Up-regulation of MICA expression was found in osteosarcoma (34/66,51.6%),compared with normal bone tissues (0/6,0%).The cell lines Saos-2,MG63,and HOS showed positive surface MICA expression,while the U2OS and OS732 showed very limited expression.[Conclusion] MICA mRNA and protein predominantly expressed on osteosarcoma tissues and cell lines.关键词
骨肉瘤/MHC/I类链相关蛋白A/MICA/表达Key words
osteosarcoma/MHC class I chain-related A/MICA/expression分类
医药卫生引用本文复制引用
卢善明,薛玲,肖萍,李扬,车丽洪,乔慧..骨肉瘤组织中MICA基因mRNA和蛋白的表达[J].中山大学学报（医学科学版）,2009,30(4):441-445,5.基金项目
国家自然科学基金(30300354) （30300354）
