果树学报2005，Vol.22Issue(5)：441-445,5.
组织培养条件下苹果Ty1-copia类逆转座子的转录活性
Transcriptiona activity of apple Ty1-copia-like retrotransposons during tissue culture
摘要
Abstract
Transcriptionally active Ty1-copia-like retrotransposons were found in apple callus cells propagated on MS solid medium supplemented with 2 mg/L of 2,4-D, 0.2 mg/L of BA and 0.5 mg/L of NAA for a month. RT-PCR was used for amplifying the conserved reverse transcriptase domain of about 270 bp. Twenty-one clones isolated from the callus cells were sequenced and showed high heterogeneity. All 21 clones were different from each other, amino acid ranging from 35.6% to 98.8%. The majority of isolated reverse transcriptase gene were found to lost their function, containing stop codons, frame shifts, deletion, and insertion mutations. The SLYGLKQ, characteristic of plant retrotransposons, contained base substitution mutation in 7 clones. Horizontal transmission of plant retrotransposons had occurred in the past. Searching through the protein GeneBank database, all 21 sequenced clones showed high homology to Ty1-copia-like elements belonging to other plant species. A phylogenetic tree was constructed based on their predicted amino acids. All sequences were divided into three groups, except ART20. Some Ty1-copia-like copies, present in apple, were found to be high methylated. In conclusion, retrotransposons characteristics have contributed to the genetic diversity and evolution of their host species.关键词
苹果/Ty1-copia类逆转座子/转录活性/组织培养Key words
Apple/Ty1-copia-like retrotransposons/Transcription/Tissue culture分类
农业科技引用本文复制引用
孙俊,房经贵,陶建敏,蔡斌华,章镇..组织培养条件下苹果Ty1-copia类逆转座子的转录活性[J].果树学报,2005,22(5):441-445,5.基金项目
国家自然科学基金资助项目(30200190). （30200190）
