中国当代儿科杂志2008，Vol.10Issue(4)：441-446,6.
雄激素对缺氧缺血性脑损伤新生大鼠脑组织芳香化酶和神经生长因子表达的影响
Effects of androgen on the expression of brain aromatase cytopigment and nerve growth factor in neonatal rats with hypoxic-ischemic brain damage
摘要
Abstract
Objective To study the effects of androgen on the expression of aromatase eytopigment P450(AROM)and nerve growth factor(NGF)in the brain and brain uhrastructure in neonatal rats with hypoxic-ischemic brain damage (HIBD)in order to investigate the mechanism underlying the protective effect of androgen against HIBD.Methods Ninty.six seven-day-old Sprague-Dawley rats were randomly divided into three groups:sham-operation,HIBD and androgen treatment(n=32 each).HIBD was induced by the ligation of left common carotid artery and hypoxia exposure.The rats in the androgen treatment and the HIBD groups were injected intraperitoneally with testosterone propionate(25 mg/kg)and arachis oil respectively immeadiatedly after hypoxia-ischemia(HI).After 24 and 72 hrs and 7 and 10 days of HI,AROM and NGF expression in the cortex and the hippoeampus was detected with the immunohistochemical method.The uhrastruetural changes of neurons in the cortex and the hippocampus were observed under a transmission electron microscope.Results Nerve cells of the HIBD group showed obvious injuries including cell organ decreasing,cellularoedema,nuclear swelling,chromatic agglutination,mitoehondria decreasing and swelling,as well as an increase in apoptotic cells.Compared with the HIBD group.the nerve cells in the androgen treatment group had integrated nuclear membrane,well-distributed ehromatin and abundant cell organs,and less cetl apoptosis and increased axon regeneration.There was a positive expression of NGF and AROM in the brain cortex and the hippocampus in the HIBD group 24 hrs after HI.The exDression of NGF and AROM increased significantly 72 hrs after HI,peaked 7 days after HI and then began to decrease but remained at a higher level than that in the sham-operation group 10 days after HI.The NGF and AROM expression in the cortex and the hippocampus in the androgen treatment group was significantly higher than that in the sham-operation and the HIBD groups 72 hrs,and 7 and 10 days after HI.Conchrsions Androgen treatment can promote axon regeneration and morphous recovery of nerons and decrease neural apoptosis in neonatal rats with HIBD.The neuroleIrotection of androgen is produced possibly through an increase in the expression of NGF and AROM in the brain.关键词
脑缺氧/缺血/雄激素/芳香化酶/神经生长因子/新生大鼠Key words
Brain hypoxia/ischemia/Androgen/Aromatase/Nerve growth factor/Neonatal rats分类
医药卫生引用本文复制引用
李占魁,沈凌,柯华,李飞,倪黎明,李清红..雄激素对缺氧缺血性脑损伤新生大鼠脑组织芳香化酶和神经生长因子表达的影响[J].中国当代儿科杂志,2008,10(4):441-446,6.基金项目
National Natural Science Foundation of China(No.30471827)and Shanxi Province Natural Science Foundation(No.2004C263) （No.30471827）
