中国输血杂志2009，Vol.22Issue(6)：443-446,4.
不同细胞因子组合对脐血造血干细胞体外扩增效果分析
Effect of different human recombinant cytokine combinations on the expansion of cord blood haemopoietic stem cells
摘要
Abstract
Objective To study the expansion efficiency of cord blood haemopoietic stem cells when cultured with dif-feront human recombinant cytokine combinations. Methods Mononuclear cells separated from cord blood were cultured in Iscove Modified Dulbccoo's medium(IMDM). Seven experimental groups (A——-G) were set up according to the addition of different cytokine combinations. Mononuclear cells were counted by trypan blue exclusion and the CD34+ cells were sorted by flow eytometry on the 3rd, 7th, and 14th culture day. Results Compared with the control group, the amount of mononu-clear cells in each experimental group increased significantly. Except group F, which reached the peak expansion of (15.60 ± 10. 90) × 109/L, on the 14th culture day, all other six groups showed peak expansion on the 7th day, among which group E was the highest [(17. 11±6. 12) × 109/L]. The percentage of CD34+ cells in group A, D, E, F, and G peaked on the 7th culture day, and the peak expansion in group B and C was observed on the 14th culture day. The number of CD34+ cells in unit volume among the seven groups ranged from (34. 7 ± 10. 4) × 107/L (group A) to ( 168.6 ± 43. 5 ) × 107/L (group E), with 10——50 folds of expansion. Conclusion All the experimental groups with different human re-combinant cytokine combinations could improve the expansion efficiency of cord blood mononuclear cells and CD34+ cells. The group with the combination of SCF + IL-3,-6 ,-2,-4 achieved the peak expansion on the 7th culture day.关键词
脐血/造血干细胞/单个核细胞/CD34+细胞/扩增Key words
Cord blood/ Haemopoietic stem cell/ Mononuclear cell/ CD34 + / Expansion分类
医药卫生引用本文复制引用
孟庆宝,魏亚明,张印则..不同细胞因子组合对脐血造血干细胞体外扩增效果分析[J].中国输血杂志,2009,22(6):443-446,4.基金项目
深圳市科技局资助项目,项目编号:No 200702107 （）
