中国现代医学杂志2004，Vol.14Issue(1)：44-46,49,4.
菌丝相和酵母相白念珠菌ERG11基因部分序列差异性的研究
Differences in parts of ERG11 gene sequences between the yeast- form and hyphal- form of candida albicans
石婧 1张宏1
作者信息
- 1. 暨南大学附属华侨医院,皮肤科,广东,广州,510632
- 折叠
摘要
Abstract
Objective: To explore the differences in gene sequences between yeast -form and hyphal -form ofCandida albicans, the sequences of ERG11 gene encoding the target enzyme of fluconazole were compared. Methods:Seven C. albicans isolates from the same strain ( CA - 10、CA - 11 、CA - 13 ～ CA - 17 ) were induced to form pseudohyphae. The genomic DNA of both the yeast - form and hyphal - form of the seven isolates were extracted. DNA fragments ( position 438 to position 701 ) of ERG1 1 were amplified by PCR and DNA differences between both forms of each isolate were compared by DN.A sequencing. Results:No isolate was found to have sequence difference between yeast form and hyphal-form in this fragment of ERG11 gene of C. albicans. Conclusion:The hyphal- form of C. albicans has no difference in parts of DNA sequence with the yeast-form in this fragment of ERG11 gene of C. albicans.关键词
白念珠菌/菌丝相/酵母相/差异/ERG11基因Key words
candida albicans/hyphal -form/yeast -form/ERG11 gene分类
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石婧,张宏..菌丝相和酵母相白念珠菌ERG11基因部分序列差异性的研究[J].中国现代医学杂志,2004,14(1):44-46,49,4.
