中山医科大学学报2001，Vol.22Issue(1)：44-47,4.
洛沙坦抑制平滑肌细胞增殖和神经肽Y作用的关系
The Inhibiting Effects of Losartan on Proliferation of Cultured Arterial Smooth Muscle Cell and Its Relationship with Neuropeptide Y
高聪 1刘健康 2黄少华 3陈敏生1
作者信息
- 1. 广州医学院 第二附属医院神经内科,
- 2. 广州医学院 组织胚胎学教研室,
- 3. 广州医学院 第一附属医 院心内科,
- 折叠
摘要
Abstract
【Objective】 To explore the mechanism of action of Losa rtan on vascular remodelling in hypertension and its relationship with neuroendo crine factor and renin-angiotensin system.【Methods】 The study consisted of co ntrol group and other three treatment groups: Losartan group、NPY group、and Los artan+NPY group. More than 200 cells were scanned in each group. The methods of c ellular culture, biochemistry and quantitative immunocytochemistry through ACAS5 70 were applied to investigate the proliferation of vascular smooth muscle cell (VSMC) and the expression of proliferating cell nuclear antigen (PCNA) in cultur ed rat VSMC treated with Losartan and Neuropeptide Y (NPY) stimulation. 【Result s】 VSMC proliferation (by absorbauce) in the control group and other three exam ed groups: Losartan group、NPY group and Losartan+NPY group were 0.223 9±0.00 1 0、0.204 5±0.001 3、0.262 6±0.002 5、0.244 0±0.001 3, and PCNA (by fl uorescence intensity) were 1 543±200、1 339±233、1 649±233、1 545±256. It wa s observed that losartan could inhibit the VSMC proliferation in vitro cultu re with and without NPY simulation. Compared with the control groups, the VSMC p roliferation activity and expression of PCNA were obviously descreased in the lo sartan treated gro ups. 【Conclusion】 The results demonstrate that losartan has the inhibitive eff ects on VSMC profiferation and PCNA expression. The results also suggest that lo sartan has anti-NPY effect on VSMC in vascular remodelling of hypertensive vess els.关键词
洛沙坦/药理学/神经肽Y/药理学/肌,平滑,血管/药物作用/免疫荧光定量技术分类
医药卫生引用本文复制引用
高聪,刘健康,黄少华,陈敏生..洛沙坦抑制平滑肌细胞增殖和神经肽Y作用的关系[J].中山医科大学学报,2001,22(1):44-47,4.
