YAN Ji-yong 1HE Yu-ke 2CAO Jia-shu3
作者信息
- 1. Institue of Vegetable Science, Zhejiang University, Hangzhou 310029, P.R. China
- 2. Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R. China
- 3. State Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Econology, Chinese Academy of Sciences, Shanghai 200032, P.R. China
- 折叠
摘要
Abstract
Three main parameters were selected to study their importance in transformation by bud microinjection in non-head Chinese cabbage [Brassica campestris ssp. Chinensis (L.) Makino var. Communis Tsen et Lee]. The results showed that the developmental stage of floral bud, the concentrations of sucrose and surfactant Silwet L-77 were critical for the successful transformation by this new method. The suitable bud size is 2- 3 mm in length, the favorable concentration of sucrose and surfactant Silwet L-77 are 8 and 0.02% respectively. When the sucrose concentration was greater than 10% or that of Silwet L-77 was above 0.1%, the treated buds became yellow and finally blighted. 4/6 T1 seedlings resistant to kanamycin were positive by PCR analysis, and T2 progeny of all these positive T1 plants have one or more hybridizing bands by Southern blot. Under 5% sucrose, 0.02% Silwet L-77 and grade 2 bud (2- 3 mm in its length) parameters, the most favorable transformation efficiency is about 0.56%, and mean efficiency reaches 0.16% in all experiments indicating that bud microinjection is potential transformation way in non-head Chinese cabbage.关键词
Brassica campestris ssp. chinensis (L.) Makino var. communis Tsen et Lee, Agrobacterium tumefaciens, Bud microinjection, Bud size, Sucrose, Silwet L-77Key words
Brassica campestris ssp. chinensis (L.) Makino var. communis Tsen et Lee, Agrobacterium tumefaciens, Bud microinjection, Bud size, Sucrose, Silwet L-77分类
农业科技
