四川大学学报（自然科学版）2000，Vol.37Issue(3)：446-450,5.
甘薯储藏蛋白(sporamin)A基因在大肠杆菌中的表达研究
EXPRESSION OF SPORAMIN A GENE OF SWEET POTATO IN ESCHERICHIA COLI
摘要
Abstract
The coding sequence of mature sporamin A of sweet patato was amplffied by PCR and inserted into the expression vector pTrcHisB , resulting in the recombinant plasmid pTHSPO-A, which was confirmed by restriction and PCR analysis . The pTHSPO-A was transformed into Escherichia coli TOP10 . After induction with IPTG ,an additional protein band with MW of 10 kD was observed by SDS-PAGE.关键词
甘薯储藏蛋白/基因表达/大肠杆菌/SDS-PAGEKey words
sporamin A/gene expression/Escherichia coli/SDS-PAGE分类
生物科学引用本文复制引用
封树梅,张义正..甘薯储藏蛋白(sporamin)A基因在大肠杆菌中的表达研究[J].四川大学学报（自然科学版）,2000,37(3):446-450,5.基金项目
四川省“九五”生物技术育种攻关项目 （）
