水生生物学报2009，Vol.33Issue(3)：449-454,6.DOI:10.3724/SP.J.0000.2009.30449
微波蒸馏-顶空固相微萃取-气质联用检测鱼体中土霉昧化合物
ANALYSIS OF OFF-FLAVORS IN FISH BY MICROWAVE MEDIATED DISTILLATION WITH HEADSPACE SOLID-PHASE MICROEXTRACTION AND GAS CHROMATOGRAPHY-MASS SPECTRUM
摘要
Abstract
Aquaculture the rearing of fish and other aquatic animals is the best approach for meeting the increasing demand for aquaculture products worldwide. However, the problem of off-flavor has been documented as a serious setback to aqua-culture. Though there are many off-flavors can result in a fish being objectionable, approximately 80% of the rejected fish have the earthy-musty odor, which is primary caused by the presence of either 2-methylisoborneol (2-MIB) or geosmin. Based on the fact that the detection of earthy-musty odorous compounds in fish mainly relied on the sensory method in Chi-na, the present study established the instrumental method to detect 2-MIB and geosmin by microwave mediated distillation with headspace solid-phase microextraction and gas chromatography-mass spectrum (MWDE-SPME-GC-MS). Through in-vestigating the effects of the key parameters, microwave processing time and carrier gas flow rate, in microwave mediated distillation on extracts,it is concluded that 6 min of microwave time and 70 mL/min nitrogen flow were the optimized con-dition on which earthy-musty odorous compounds could be completely distilled from fish flesh. Then the earthy-musty odor-ous compounds were absorbed in the fiber under HS-SPME, and were finally desorbed at 250℃ and determined by GC-MS. The limits of detection for 2-MIB and geosmin in fish were both 0.1 μg/kg. There were significant linear correlation in the range of 1-20 μg/kg for the two earthy-musty odorous compounds, with calibration coefficients at 0.987 and 0.995, respectively. Therefore, trace levels of earthy-musty odorous compounds at ppb in fish could be quantified by this method with credible results.关键词
微波蒸馏/顶空固相微萃取/气质联用/土霉味/2-甲基异茨醇/土腥素Key words
Microwave mediated distillation/Headspace solid-phase microextraction/GC-MS/Off-flavors/2-Methyli-soborneol/Geosmin分类
生物科学引用本文复制引用
张婷,李林,陈伟,宋立荣..微波蒸馏-顶空固相微萃取-气质联用检测鱼体中土霉昧化合物[J].水生生物学报,2009,33(3):449-454,6.基金项目
中国科学院知识创新工程重要方向性项目(No.KZCX2-YW-426) （No.KZCX2-YW-426）
湖北省"十一五"科技攻关项目(No.2006AA203A02)资助 （No.2006AA203A02）
