中国现代医学杂志2001，Vol.11Issue(4)：4-5,2.
16S-23S rDNA间隔区序列寡核苷酸探针对龟分枝杆菌暴发感染的鉴定
THE IDENTIFICATION OF POSTOPERATIVE M. CHELONAE INFECTIIONOUTBREAK BY OLIGONUCLEIED PROBE OF 16S- 23S rDNA SPACER SEQUENCE
张灵霞 1庄玉辉 1扈庆华2
作者信息
- 1. 中国人民解放军第309医院结核病研究室
- 2. 深圳市卫生防疫站
- 折叠
摘要
Abstract
To determine the pathogen of the infection outbreak in Shen Zhen and He bei at gene level with molecular biological technique, and to establish a rapid identification method of M. chelonae subsp. abscessus by dot blot. We designed a oligonucleitide probe and the sensitivity and specificity of the probe were studied. The results showed: the sensitivity of the probe detecting PCR products was 1ng. Only M. chelonae subsp. abscessus was hybridization with the probe, the positive rate of the probe detecting clinical specimen was 66.6%.关键词
龟分枝杆菌暴发感染寡核苷酸探针PCR16S-23SrDNA间隔区分类
医药卫生引用本文复制引用
张灵霞,庄玉辉,扈庆华..16S-23S rDNA间隔区序列寡核苷酸探针对龟分枝杆菌暴发感染的鉴定[J].中国现代医学杂志,2001,11(4):4-5,2.
