重庆医学2001，Vol.30Issue(1)：4-5,2.
一氧化氮对内毒素血症大鼠肠道损伤及细菌移位的影响
Effect of nitric oxide on intestinal damage and bacterial translocation in endotoxemic rats
肖南 1刘韧 1田昆仑 1刁有芳 1刘宝华 1陈惠孙1
作者信息
摘要
Abstract
Objective The effects of nitric oxide(NO) on the endotoxin-induced tissue damage,especially intestinal injury and bacterial translocation are still poorly known, although its involvement in vasodilatation and hypotenion in shock is much clear. So, the intestinal damage and bacterial translocation were observed in this study in endotoxemic rats after treatment with Nω-nitro-L-arginine(LNNA),the special inhibitor of NO synthase(NOS),and L-arginine,the substrate of NOS.Methods The endotoxemia was conducted with administration of lipopolysaccharide(O111B4,10mg/kg,i.p.),animals were treated with LNNA(4mg/kg,i.p.)or L-arg(40mg/kg,i.p.).Intestinal molondialdehyde(MDA) content and Diamine oxidase(DAO) activity were determined,and mesenteric lymph nodes were cultured.Results The results showed that endotoxin decreased intestinal DAO acitivity but increased MDA content and incidence of bacterial translocation to mesenteric lymph nodes.These effects of endotoxin were aggregated by inhibition of NO production with LNNA, but attenuated by L-arg.Conclusion We concluded that inhibition of NO formation might enhance endotoxin-induced intestinal damage and bacterial translocation,which suggested that NO might play a protective role in this endotoxemia model.关键词
一氧化氮/内毒素血症/细菌移位分类
医药卫生引用本文复制引用
肖南,刘韧,田昆仑,刁有芳,刘宝华,陈惠孙..一氧化氮对内毒素血症大鼠肠道损伤及细菌移位的影响[J].重庆医学,2001,30(1):4-5,2.
