农业生物技术学报2004，Vol.12Issue(4)：450-454,5.
曲霉Aspergillus sojae壳聚糖酶的诱导及特性
Induction and Some Properties of Chitosanase from Aspergillus sojae
摘要
Abstract
After chitosanase induction in the optimal culture conditions of Aspergillus sojae, Czapek-Dox medium (CD) +2%N-acetylglucosamine (GlcNAc)+1% peptone+0.5% yeast extract, 26℃ and 150 r/min for 60 h, the chitosanolytic activity reached a maximum level (12.34 mU/mL), and four isoenzymes were detected by active staining after SDS-PAGE, CHA1, CHA2, CHA3 and CHA4, with molecular weights of about 38.2, 30.8, 27.7 and 25.0 kD, respectively. Some properties showed the chitosanolytic enzyme was an endo-chitosanase. Analysis of reaction products by thin-layer chromatography (TLC) indicated that the chitosanase hydrolyzed chitosan 10B(D.A.0%) into chitobiose. The chitosanase cleaved chitosan 7B (D.A.30%) with the maximum velocity and the activity declined with decreasing degree of acetylation (D.A.) of chitosan-type substrates,but difficultly hydrolyzed chitin-type substrates.关键词
Aspergillussojae/壳聚糖/壳聚糖酶/诱导/特性Key words
Aspergillus sojae,, chitosan/chitosanase/induction/characteristic分类
农业科技引用本文复制引用
张学昆,李加纳,陆合..曲霉Aspergillus sojae壳聚糖酶的诱导及特性[J].农业生物技术学报,2004,12(4):450-454,5.基金项目
Supported by the State High Science and Technology Program(863) of China. （863）
