高等学校化学学报2001，Vol.22Issue(3)：450-454,5.
肌红蛋白在双十二烷基二甲基溴化铵-粘土 多双层复合薄膜电极上的电化学与电催化
Electrochemistry and Electrocatalysis with Myoglobin at DDAB-clay Multibilayer Composite Film Electrodes
摘要
Abstract
Mb-DDAB-clay film electrodes were made by casting aqueous dispersions of didodecyldimethylammonium bromide(DDAB)-clay composite and the protein myoglobin(Mb) on pyrolytic graphite(PG) electrodes. Cyclic voltammetry of Mb-DDAB-clay films showed a pair of well-defined and nearly reversible peaks at about -0.25 V vs. SCE in pH 5.5 buffers, being the characteristic peaks of the Mb heme Fe(Ⅲ)/Fe(Ⅱ) redox couples. In the microenvironment of DDAB-clay films, the electron transfer between Mb and PG was greatly enhanced, and showed a good stability during storage. Soret absorption band positions suggested that Mb retained its native conformation in these films at medium pH. The interlayer clay spacing estimated by X-ray diffraction suggested that the incorporated Mb did not disturb the ordered multilayer structure of the films. The average formal potential of Mb heme Fe(Ⅲ)/Fe(Ⅱ) couples in DDAB-clay films shifted linearly from pH 4.5 to 11 with a slope of -53 mV/pH, suggesting that one proton was coupled with each electron transfer. The dissolved oxygen and trichloroacetic acid was catalytically reduced by Mb-DDAB-clay film electrodes.关键词
肌红蛋白/表面活性剂-粘土复合物/多双层复合薄膜电极/电化学/电化学催化分类
化学化工引用本文复制引用
胡乃非,李溱,马红艳..肌红蛋白在双十二烷基二甲基溴化铵-粘土 多双层复合薄膜电极上的电化学与电催化 [J].高等学校化学学报,2001,22(3):450-454,5.基金项目
国家自然科学基金 （）
