中国农业科学(英文版)Issue(1)：45-49,5.
The Cloning and Sequencing of Read-through Protein Gene from BYDV-GAV Virus
The Cloning and Sequencing of Read-through Protein Gene from BYDV-GAV Virus
摘要
Abstract
The cDNA of BYDV-GAV read-through protein (RTP) gene was amplified from the extracted RNA of BYDV-GAV by using the polymerase chain reaction (PCR), and cloned into pGEM-7zf( + ). Its complete nucleotide sequence was determined by dideoxynucleotide chain-termination method. The BYDV-GAV RTP gene consists of 1377nt. Its sequences were most similar to that of the RTP gene of BYDV - MAV with identities of 87.4% and 87.1% at the nucleotide and amino acid levels, respectively.关键词
BYDV-GAV/Read-through protein gene/SequenceKey words
BYDV-GAV/Read-through protein gene/Sequence分类
农业科技引用本文复制引用
CHANG Sheng-jun,WANG Xi-feng,LI Li,MA Zhan-hong,ZHOU Guang-he..The Cloning and Sequencing of Read-through Protein Gene from BYDV-GAV Virus[J].中国农业科学(英文版),2001,(1):45-49,5.基金项目
This work is supported by the National Key Basic Research (Project 973 No. G2000016201), and National Natural Science Foundation of China (39970034). （Project 973 No. G2000016201）
