中国组织工程研究与临床康复2008，Vol.12Issue(23)：4554-4558,5.
聚羟基丁酸/羟基戊酸共聚酯构建引导骨组织再生膜
Poly (hydroxybutyrate-hydroxyvalerate) membrane for guiding bone regeneration in vivo
摘要
Abstract
BACKGROUND: The shape and amount of bone regenerated depend on the existence and maintenance of hypolemmal space. Collapse and reduction of hypolemmal space will greatly inhibit bone regeneration. Therefore, the key to success of induced bone regeneration is to select membrane material.OBJECTIVE: This study is designed to investigate the feasibility of poly (hydroxybutyrate-hydroxyvalerate) (PHBV) membrane for guiding bone regeneration.DESIGN, TIME AND SETTING: This study, a self-control animal experiment, was performed at the Laboratory Animal Center, Third Hospital Affiliated to Sun Yat-sen University between May 2005 and October 2006.MATERIALS: PHBV membrane, (10×10×0.3)mm, was provided by Research of Biomaterials, College of Material Science and Engineering, South China University of Technology. Eight healthy hybrid dogs, aged 1 year old, weighing 10-12kg, were included for model of tibial defects.METHODS: Four bone defects were made on bilateral tibias in each dog. Bilateral proximal bone defects of all dogs were included as control group and only subjected to periosteal flap reduction. Bilateral distal bone defects of all dogs were included as experimental group and covered by PHBV membrane. Samples were harvested at weeks 2, 4, 8, and 12 post-surgery.MAIN OUTCOME MEASURES: In vivo degradation performance and ossific capability of PHBV copolymer as well as energy-dispersive X-ray analysis and elemental quantitative assay in bone defect regions.RESULTS: In the experimental group, at week 8 post-surgery, PHBV copolymer membrane had been gradually degraded, different sizes of hollows formed on its surface, bone defect surface was smooth and flat, and X-ray results showed increased bone density in the bone defect regions; at week 12 post-surgery, the PHBV copolymer was still not degraded completely, but bone defect regions had been completely fused with normal bone. Energy-dispersive X-ray analysis and elemental quantitative assay results demonstrated in the experimental group, peaks of Ca and P atom number of newly formed bone were predominant, and peaks of impurity were few. With time going, element Ca and P levels in the newly formed bone were increased, the ratio for Ca and P atoms approximated to that in the normal bone. There was significant difference between the. experimental group and the control group (P<0.05).CONCLUSION: PHBV copolymer membrane is a promising ideal natural membrane material for guiding bone regeneration.关键词
丁酸类,聚合物/引导骨再生/骨缺损/生物降解/生物材料分类
医药卫生引用本文复制引用
唐倩,陈建洪,粱焕友,吴坚,李源,陈晓峰,吴刚..聚羟基丁酸/羟基戊酸共聚酯构建引导骨组织再生膜[J].中国组织工程研究与临床康复,2008,12(23):4554-4558,5.基金项目
广东省科技计划项目(2005B30901010) （2005B30901010）
广东省医学科研基金(A2003049) Science and Technology Plan Project of Guangdong Province, No. 2005B30901010 （A2003049）
Medical scientific Research Foundation of Guangdong Province, China, No. A2003049 （）
