中华医学杂志（英文版）2002，Vol.115Issue(3)：455-45,2.
C6脑胶质细胞瘤的在体实验研究
Development of a rat C6 brain tumor model
摘要
Abstract
Objective To establish rat C6 brain-tumor models and find a simple reliable index to judge tumor volume. Methods C6 cell suspension (10μl) containing 10g/L agarose and 1×106 cells was injected into the right caudate nucleus of the rat brain by a stereotaxic method. After implantation, rats were observed and given MRI scans. Rats were perfused with paraformaldehyde trans-aorta at the 10th, 15th, 20th and 25th day and before natural death. All brains, lungs, spinal cords and tumors were sectioned and inspected. Tumor-containing samples were prepared histologically by hematoxylin and eosin (HE) stains. Results Fifty implanted rats had 100% yield of intracerebral growth, with distant metastasis of 0% to 4%. Conclusion A rat C6 brain-tumor model was successfully established. Rat survival time is correlated with tumor volume and may be useful as an index of tumor size.z关键词
C6脑胶质瘤细胞 立体定向移植 大鼠 脑Key words
C6 glioma cell * stereotaxic implantation * rat * brain分类
医药卫生引用本文复制引用
章翔,吴景文,高大宽,费舟,屈延,荆俊杰..C6脑胶质细胞瘤的在体实验研究[J].中华医学杂志（英文版）,2002,115(3):455-45,2.基金项目
This study was supported by a grant from the National Natural Science Foundation of China (No. 39970752). （No. 39970752）
