青岛大学医学院学报2009，Vol.45Issue(5)：455-458,4.DOI:10.3969/j.issn.1672-4488.2009.05.017
小干扰RNA沉默EGFR基因对卵巢癌细胞侵袭力的影响
EFFECT OF siRNA SILENCING EGFR GENE ON INVASIVE POWER OF OVARIAN CANCER CELLS
宋慧 1陈爱平 1杨蕊蕊 1张红玲1
作者信息
- 1. 青岛大学医学院附属医院妇科,山东,青岛,266003
- 折叠
摘要
Abstract
Objective To investigate the effect of RNA interference (RNAi) on the invasion power of ovarian cancer cell line SKOV3 in vitro through the gene expression of small interference silence epidermal growth factor receptor (EGFR). MethodsThe siRNA plasmid of target EGFR gene and negative-control plasmid were constructed in vitro, which were transfected to SKOV3 cells mediated by Lipofectamin 2000. The study consisted of three groups: blank control; non-specific siRNA transfected (NS-siRNAT) and specific siRNA transfected (S-siRNAT). The expressions of EGFR mRNA and protein were detected by reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemical tecnique. The proliferation of SKOV3 cells was detected by clone formation assay and in vitro invasive power of the cells detected by Transwell assay. Results The expression in S-siRNAT group was markedly weaken than that in the orther two groups in terms of EGFR mRNA (F=50.45;q=12.87,11.72;P<0.01), the protein (F=61.54;q=14.58,11.46;P<0.01), the proliferation of the cells (F=130.12;q=21.12,18.15;P<0.01) and in vitro invasive power (F=34.09;q=11.26,8.34;P<0.01). Conclusion siRNA can effectively suppress the expression of EGFR gene and lead to the suppression of proliferation and invasiveness of SKOV3 cells.关键词
卵巢肿瘤/RNA,小分子干扰/基因,erbB-1/侵袭力Key words
Ovarian tumor/RNA interference/Gene, erbB-1/Invasive power分类
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宋慧,陈爱平,杨蕊蕊,张红玲..小干扰RNA沉默EGFR基因对卵巢癌细胞侵袭力的影响[J].青岛大学医学院学报,2009,45(5):455-458,4.
