首页|期刊导航|中华医学杂志（英文版）|Effects of ketamine and midazolam on morphology of dendritic spines in hippocampal CA1 region of neonatal mice
中华医学杂志（英文版）2009，Vol.122Issue(4)：455-459,5.DOI:10.3760/cma.j.issn.0366-6999.2009.04.0018
Effects of ketamine and midazolam on morphology of dendritic spines in hippocampal CA1 region of neonatal mice
Effects of ketamine and midazolam on morphology of dendritic spines in hippocampal CA1 region of neonatal mice
摘要
Abstract
Background It is a common phenomenon that children experience multiple general anesthesias in clinical practice, which raises the question whether repeated exposure to general anesthetics would interfere with the development of the central nervous system of children. The present study was designed to evaluate the effects of repeated treatment with ketamine or midazolam on postnatal dendrite development by examining the morphology of the dendritic spines of the pyramidal neurons in the hippocampal CA1 region in mice.Methods The transgenic green fluorescent protein-M line (GFP-M) mice were used in this study. Ketamine (100 mg/kg), midazolam (50 mg/kg) or saline (10 ml/kg) was administered intraperitoneally once a day on consecutive days from postnatal day 8 (P8) to postnatal day 12 (P12). At postnatal day 13 (P13) and postnatal day 30 (P30), the density and length of the apical dendritic spines of the pyramidal neurons in the hippocampal CA1 region were examined under a confocal microscope.Results At P13, for both the ketamine group and the midazolam group, the dendritic spines were found with a comparatively lower density and longer average length than in the control group. At P30, no significant difference in the density or average length of dendritic spines was found between the anesthetic group and control group.Conclusions This study indicated that repeated exposure to ketamine or midazolam in neonatal mice impaired dendritic spine maturation immediately afterwards, but this influence seemed to disappear during further postnatal development.关键词
dendritic spines/ ketamine/ midazolam/ confocalKey words
dendritic spines/ ketamine/ midazolam/ confocal分类
医药卫生引用本文复制引用
TAN Hong ,REN Rong-rong,XIONG Zhi-qi,WANG Ying-wei..Effects of ketamine and midazolam on morphology of dendritic spines in hippocampal CA1 region of neonatal mice[J].中华医学杂志（英文版）,2009,122(4):455-459,5.基金项目
This study was supported by a grant from Shuguang Program (08SG18) of Shanghai Municipal Education of Commission. （08SG18）
