首页|期刊导航|中国农业科学（英文版）|Transgenic Rape with hrf2 Gene Encoding HarpinXooc Resistant to Sclerotinia sclerotinorium
中国农业科学（英文版）2008，Vol.7Issue(4)：455-461,7.
Transgenic Rape with hrf2 Gene Encoding HarpinXooc Resistant to Sclerotinia sclerotinorium
Transgenic Rape with hrf2 Gene Encoding HarpinXooc Resistant to Sclerotinia sclerotinorium
摘要
Abstract
The objective of this study was to increase the resistance of rape using the method of transformation. The gene hrf2 derived from Xathomonas oryzae pv. oryzicola encoding harpinXooc protein was constructed into transgenic vector pCAMBIA1301. The cotyledonal petiole segments from rapeseed variety Yangyou 4 were infected by Agrobacterium tumefaciens strain LBA4404/pCAMBIA1301-hrf2. Hygromycin-resistant green shoots were obtained. Successful integration of the foreign gene into the genome of the rapeseed variety Yangyou 4 was confirmed by PCR, RT-PCR, and β-glucuronidase analyses. Disease bioassays of transgenic plants revealed an improved resistance of transgenic plants to Rape sclerotiniose. In brief, the hrf2 gene can be transferred into rape using the method of Agrobacterium-medmted transformation, which increased the resistance to Sclerotinia sclerotinorium in the transgenic plant.关键词
hrf2 gene, Agrobacterium tumefaciens-mediated transformation, transgenic oilseed rape, resistanceKey words
hrf2 gene, Agrobacterium tumefaciens-mediated transformation, transgenic oilseed rape, resistance分类
农业科技引用本文复制引用
MA Ling-li,HUO Rong,GAO Xue-wen,HE Dan,SHAO Min ,WANG Qi..Transgenic Rape with hrf2 Gene Encoding HarpinXooc Resistant to Sclerotinia sclerotinorium[J].中国农业科学（英文版）,2008,7(4):455-461,7.基金项目
This project was supported by the National High Technology Research and Development Program of China(2006AA10Z172)and the National Special Program for Research and Industrialization of Transgenic Plants(JY03-B-12). （2006AA10Z172）
