中国组织工程研究与临床康复2008，Vol.12Issue(23)：4563-4567,5.
新型多孔β-磷酸三钙作为骨组织工程支架材料的评价
New porous beta-tricalcium phosphate as a scaffold for bone tissue engineering
摘要
Abstract
BACKGROUND: New porous β-tricalcium phosphate (TCP) was made by appropriate prescription and unique technology, with a porosity of (75±10)%, spheroidal hole>80%, micropore<20%, interlink rate between the holes of 100% and mechanical strength>2MPa.OBJECTIVE: To assess the application outcome of the new porous β-TCP as a scaffold for bone tissue engineering.DESIGN, TIME AND SETTING: The control experiment was performed at the Laboratory of Tissue Engineering of Southern Medical University, China from July 2005 to March 2006.MATERIALS: Twelve 6-month New Zealand rabbits were used to create 1.5cm large bone and periosteum defects of the left radial bone. Porous β-TCP was purchased from bio-lu, France.METHODS: Osteoblasts differentiated from rabbit bone marrow mesenchymal stem cells (BMSCs) were co-cultured with porous β-TCP. Inverted phase contrast microscope and scanning electron microscope were used to observe the growth of BMSCs. MTT assay was employed to assess cell proliferation and compatibility. Cytotoxicity was detected by analyzing the effects of different concentrations of porous β-TCP leaching liquor on cell proliferation.MAIN OUTCOME MEASURES: Cell compatibility and cytotoxicity of β-TCP were measured. The status of bone defect repair was appraised by histology, radionuclide bone scan and X-ray at 2, 6, 12 weeks after surgery.RESULTS: The new porous β-TCP had good cell adhesion and its cytotoxicity was in 0 grade. Histology, imageology and radionuclide bone scan showed the new porous β-TCP could repair large radial bone defect in rabbits. At the same time, its degradation rate was accordance with bone formation rate in vivo.CONCLUSION: The new porous β-TCP with a good compatibility is a good scaffold for bone tissue engineering, and obtains good outcomes in repairing large bone defect of rabbit radial bone.关键词
β-磷酸三钙/生物相容性/细胞毒性/降解/骨组织工程/生物材料分类
医药卫生引用本文复制引用
刘勇,裴国献,江汕,任高宏..新型多孔β-磷酸三钙作为骨组织工程支架材料的评价[J].中国组织工程研究与临床康复,2008,12(23):4563-4567,5.基金项目
国家高技术研究发展计划(八六三)资助项目(2003AA205001) （八六三）
国家自然科学基金资助项目(30600643) the National High Technology Research and Development Program or China (863 Program), No. 2003AA205001 （30600643）
the National Natural Science Foundation of China, No. 30600643 （）
