中国当代儿科杂志2004，Vol.6Issue(6)：456-461,6.
川崎病患儿外周血基质金属蛋白酶-9的表达及其与冠状动脉损伤的关系
Matrix metalloproteinase-9 expression in the peripheral blood of children with Kawasaki disease and its relationship with coronary artery lesions
杨世伟 1王大为 1秦玉明 1李军 1王凤鸣 1左维嵩1
作者信息
- 1. 南京医科大学附属南京儿童医院心内科,江苏,南京,210008
- 折叠
摘要
Abstract
Objective Matrix metalloproteinase-9 (MMP-9), a metalloproteinase, is capable of degrading type Ⅳ,V collagens, as well as gelatins. Increased levels of MMP-9 have been detected in aortic aneurysms in adult human,suggesting its role in arterial wall destruction and aneurysm formation. This study was designed to investigate the potential role of MMP-9 in the pathogenesis of coronary artery lesions in Kawasaki disease (KD) patients. Methods Twentyseven children with KD [17 with coronary artery lesions (CALs) and 10 without] and age-matched 10 febrile and 10 healthy controls were enrolled in this study. Gelatin zymography and ELISA were used to detect the activity and levels of serum MMP-9. MMP-9 mRNA expression in the leucocytes was detected using reverse transcription-polymerase chain reaction (RT-PCR). Results 1) The activity (10.2 ± 2.2) and levels of MMP-9 (1 116 ± 691 ng/ml) in KD patients withCALs in the acute phase were significantly higher than those without CALs (6.2 ± 2.1, 457 ± 133 ng/ml,respectively; P ＜0.05). Both of them in either the KD patients with CALs or without were higher than those of the healthy controls (0.1 ± 0.0, 72 ± 24 ng/ml, respectively; P ＜ 0.01) and the febrile controls (3.1 ± 1.4,221 ± 154 ng/ml, respectively; P ＜0.05). 2) There was a significantly positive correlation between the serum MMP-9 protein levels and the circulating leucocytes counts in KD patients in the acute phase ( r =0.480, P ＜0.05). 3) The MMP-9 mRNA expression in the leucocytes of KD patients in the acute phase were significantly elevated, as compared with the febrile and healthy controls ( P ＜0.01). There were no significant differences in the MMP-9 mRNA expressions between the two KD groups. 4) The activity, protein levels and mRNA expression of MMP-9 in the KD patients decreased obviously from the subacute through the convalescent phases, as compared with the acute phase ( P ＜0.01). Conclusions The MMP9 expression in KD patients in the acute phase was significantly elevated, especially in those with CALs. MMP-9 may be involved in the development of coronary artery lesions in KD.关键词
川崎病/冠状动脉病变/基质金属蛋白酶-9Key words
Kawasaki disease/Coronary artery lesions/Matrix metalloproteinase-9分类
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杨世伟,王大为,秦玉明,李军,王凤鸣,左维嵩..川崎病患儿外周血基质金属蛋白酶-9的表达及其与冠状动脉损伤的关系[J].中国当代儿科杂志,2004,6(6):456-461,6.
