首页|期刊导航|中德临床肿瘤学杂志（英文版）|The study of RNAi-mediated by conditionally replicating adenovirus silencing on Survivin gene in colon cancer cell lastingly
中德临床肿瘤学杂志（英文版）2008，Vol.7Issue(8)：460-464,5.
The study of RNAi-mediated by conditionally replicating adenovirus silencing on Survivin gene in colon cancer cell lastingly
The study of RNAi-mediated by conditionally replicating adenovirus silencing on Survivin gene in colon cancer cell lastingly
摘要
Abstract
Objective: To investigate the effect of RNAi-mediated Survivin gene with conditionally replicating adenovirus silencing on Survivin gene in colon carcinoma cell lines HT-29 lastingly.Methods: We transfected Ad-delElb55KD-shRNA/ Survivin-EGFP to HT-29 (control was replication defective adenovirus and liposome vector which was contained the same shRNA as Ad-delE1b55KD-shRNA/Survivin-EGFP).The expressions of EGFP, Survivin mRNA and Survivin protein in HT29 were detected at the 1st, 7th, 14th and 28th days after transfection.Results: The expression of EGFP, the inhibition of Survivin mRNA and Survivin protein in HT-29 were high in each group at the 7th day after transfection, among the total, the effect of Ad-delE1b55KD-shRNA/Survivin-EGFP group was the highest; at the 14th day, the effects of replication defective adenovirus group and liposome vector group were decreased obviously, and it was still high in Ad-delE1b55KD-shRNA/Survivin-EGFP group; at the 28th day, the effects of control groups were disappeared, and it was still high in Ad-delE1b55KDshRNA / Survivin-EGFP group like before (P＜0.05).Conclusion: RNAi-mediated Survivin gene with conditionally replicating adenovirus can silence Survivin gene in colon carcinoma call lines HT-29 lastingly.关键词
conditionally replicating adenovirus/RNA interference/Survivin/colon cancer/apoptosisKey words
conditionally replicating adenovirus/RNA interference/Survivin/colon cancer/apoptosis分类
医药卫生引用本文复制引用
Chunyi Wang,Zhongxue Fu..The study of RNAi-mediated by conditionally replicating adenovirus silencing on Survivin gene in colon cancer cell lastingly[J].中德临床肿瘤学杂志（英文版）,2008,7(8):460-464,5.基金项目
Supported by a grant from the Nature Sciences Foundation of Chongqing (No.2005BB5225). （No.2005BB5225）
