中山大学学报（医学科学版）2009，Vol.30Issue(4)：463-467,5.
实时荧光定量 PCR检测野生型p53介导蛋白磷酸酶mRNA在非小细胞肺癌中的表达
Expression of Wip1 mRNA in Non-small Cell Lung Cancer by Real-time PCR
摘要
Abstract
[Objective] The aim of this study was to establish a quantitative SYBR Green Ⅰ real-time PCR method for detection of wide-type p53-induced phosphatase 1 (Wip1 or PPM1D) gene expression level in non-small cell lung cancer (NSCLC),and to investigate the relationship between Wip1 mRNA expression level and the clinicopathological characters.[Method] Real-time PCR was employed to determine the expression level of Wip1 mRNA in 44 specimens of NSCLC tissues and their adjacent normal tissues.[Results] In the 44 specimens,the expression of Wip1 mRNA in both cancer tissues and adjacent normal lung tissues were positive.Wip1 gene was overexpressed in 17 specimens among 44 NSCLC specimens.The rate was 38.6%.The relative level of Wip1 mRNA in NSCLC tissues was significantly higher than the adjacent normal lung tissues (Ratio ＝ 2.1644 ± 1.394,P ＜ 0.01).The expression of Wip1 mRNA was also correlated with pathological staging (F ＝ 5.08,P ＝ 0.013).[Conclusion] The established SYBR Green Ⅰ quantitative real-time PCR method can successfully detect the expression level of Wip1 mRNA.The results suggested that Wip1 may be involved in the development of NSCLC.关键词
非小细胞肺癌/Wip1基因/实时定量聚合酶链反应Key words
non-small cell lung cancer/Wip1/real time fluoresene quantitative PCR分类
医药卫生引用本文复制引用
张伟,赵吉星,罗红鹤,鲁建军,马俊,顾勇..实时荧光定量 PCR检测野生型p53介导蛋白磷酸酶mRNA在非小细胞肺癌中的表达[J].中山大学学报（医学科学版）,2009,30(4):463-467,5.基金项目
广东省医学科技基金(2007C24004) （2007C24004）
