PCR-SSP法检测人胰腺癌细胞株PCNA-1的K-ras基因点突变的方式OA
Detection of K-ras Gene Point Mutation's Style in Human Pancreatic Cancer Cell Line PANC-1 by PCR-SSP
Objective: To detect the style of K-ras gene point mutation in human pancreatic cancer cell line PANC-1 and decide the bp sequence of Ras target position interfered by RNA. Methods: Three kinds of special sequence primers (SS…查看全部>>
Objective: To detect the style of K-ras gene point mutation in human pancreatic cancer cell line PANC-1 and decide the bp sequence of Ras target position interfered by RNA. Methods: Three kinds of special sequence primers (SSP) for polymerase chain reaction (PCR) with regard to the mutation styles (GAT, CGT and GGT) at codon 12 of K-fas were used to study the human pancreatic cancer cell line PANC-1. The amplification products were studied with polyacrylamin…查看全部>>
王伟;王春友;董继华;赵刚;陈雄;张敏
Department of Pancreas Surgery Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Pancreas Surgery Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Central Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Pancreas Surgery Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, ChinaDepartment of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
临床医学
pancreatic cancerK-ras genepoint mutationpolymerase chain reaction
pancreatic cancerK-ras genepoint mutationpolymerase chain reaction
《中德临床肿瘤学杂志(英文版)》 2006 (1)
46-48,3
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