热带作物学报2006，Vol.27Issue(3)：46-50,5.
香蕉NBS-LRR抗病基因类似序列的克隆和分析
Cloning and Characterization of One NBS-LRR like Resistance Gene Analog from Banana
摘要
Abstract
Degenerated PCR using primers targeted to NBS conserved motifs is a rapid and facile strategy for isolation of NBS sequences linked to disease resistance genes. This PCR-based approach was used isolate and characterize a NBS-LRR like resistance gene analog (GenBank Accession No. AY739270) from banana, which was named B-NBS.After the multialignment with NBS domains of other six known plant R proteins, four NBS conserved domains were genetic distance of B-NBS with NBS domains of other six known plant R proteins was found smaller than 21.7. This showed that B-NBS was an NBS-LRR like resistance gene analog (RGA). This will lay a RGAs resources foundation for cloning banana R-gene and studying its disease resistance mechanism.关键词
香蕉/抗病基因/类似序列/NBS-LRRKey words
banana/R-gene/analog/NBS-LRR分类
农业科技引用本文复制引用
莫庭辉,黄俊生,杜中军,徐立,陈业渊..香蕉NBS-LRR抗病基因类似序列的克隆和分析[J].热带作物学报,2006,27(3):46-50,5.基金项目
This work was supported by China Postdoctoral Science Foundation (Contact No. 2005037628) and Science and Technology Foundation of South China Agricultural University (Contact No. Rnd0401) （Contact No. 2005037628）
