中国免疫学杂志2000，Vol.16Issue(9)：465-467,3.
白介素-1受体相关激酶-2反义寡核苷酸对IL-1和TNF诱导PGI2合成的不同影响
Different response of antisense IRAK-2 oligonucleotides to PGI2 release induced byIL-1 and TNF
李亦蕾 1郭甫坤 1吴曙光1
作者信息
- 1. 第一军医大学药物研究所，广州 510515
- 折叠
摘要
Abstract
To explore the effects of artisense IRAK-2 oligonucleotide on the prostacyclin (PGI2) synthesis in human umbilicalvein endothelial cells (HUVEC) induced by interleukin-1 (IL-1) and tumor necrosis factor (TNF).Methods:The HUVECs were transfectedwith antisense interleulkin-1 receptor associated kinase-2 oligonucleotide (IRAK-20DN) and stimulated with IL-1 and TNF. The levels of PGI2release were analyzed by competitive ELISA. Results: Pre-transfection with antisense IRAK-20DN could remarkably decrease the levels ofPGI2 synthesis induced by IL-1 in time- and dose-dependent manner, whereas it could not attenuate the one stimulated by TNF. Conclusion:The response of antisense IRAK-2 ODN to IL-1 and TNF-stimulated PGI2 release were different. IRAK-2 plays a key role in the IL-1 signalingevents leading to PGI2 release.关键词
白介素-1/受体相关激酶-2/前列腺环素/反义寡核苷酸Key words
Interleukin-1 Receptor associated kinase-2 Prostaglandin I2 Antisense oligonucleotide分类
医药卫生引用本文复制引用
李亦蕾,郭甫坤,吴曙光..白介素-1受体相关激酶-2反义寡核苷酸对IL-1和TNF诱导PGI2合成的不同影响[J].中国免疫学杂志,2000,16(9):465-467,3.
