中国当代儿科杂志2004，Vol.6Issue(6)：466-469,4.
高压氧治疗对新生大鼠缺氧缺血性脑损伤的保护作用
Hyperbaric oxygen therapy protects rats from hypoxic-ischemic brain damage
摘要
Abstract
Objective To investigate the protective effects of hyperbaric oxygen (HBO) against hypoxic ischemic brain damage (HIBD) of neonatal rats. Methods Seven-day-old Sprague-Dawley (SD) rat pups were randomly divided into 4 groups ( n = 10 each): Control group, HIBD group, Hyperbaric air (HBA) group, and Hyperbaric oxygen (HBO) group. The HIBD model was produced by permanent occlusion of left common carotid artery and followed by exposure to a mixture of 8% oxygen and 92% nitrogen for 2 hrs (at 37℃ ). HBO and HBA treatments [2 atmosphere absolute (ATA) for 1 hr] were administered once daily to rats in the HBO and HBA group respectively after hypoxia for 7 days. Radial arm maze and sensorimotor functional tests were administered from 30 to 35 postnatal days. At the end of the behavior trials, the rats were sacrificed and cerebral histology was analyzed. The CAi subfield neurons numbers were counted to evaluate the brain damage. Results In the behavior test, the HIBD group showed different degrees of neurological damage. HBO treatment resulted in significant protection against hypoxia-ischemia induced behavior impairments (all P ＜ 0.01 ). However, the HBA group did not show any significant improvement. There was a significant reduction of CA1 neuron density in the left hemisphere of HIBD and HBA groups, compared with that of the Control group and the HBO group (all P ＜ 0.01). Conclusion HBO therapy can attenuate brain damage and improve learning, memory and sensorimotor functions in neonatal rats after HIBD.关键词
缺氧缺血,脑/高压氧/行为/大鼠,新生Key words
Hypoxia-ischemia, brain/Hyperbaric oxygen/Behavior/Rat, neonatal分类
医药卫生引用本文复制引用
余小河,杨于嘉,王霞,钟乐..高压氧治疗对新生大鼠缺氧缺血性脑损伤的保护作用[J].中国当代儿科杂志,2004,6(6):466-469,4.基金项目
Supported by Department of Public Health of Hunan Province (No. 00024). （No. 00024）
