吉林农业大学学报2008，Vol.30Issue(4)：466-471,6.
美洲板栗愈伤组织诱导和植株再生的研究
In Vitro Callus Induction and Shoot Initiation of American Chestnut (Castanea dentata)
摘要
Abstract
In vitro callus induction and shoot initiation of the American chestnut ( Castanea dentata ) were investigated in response to different plant growth regulators (auxins and cytokinins) in the culture medium. Micropropagated shoots were used as explant materials. Auxins included indolebutyric acid (IBA), naphthaleneacetic acid (NAA), 2, 4-dichlorophenoxyacetic acid ( 2, 4-D ), and 2, 4, 5- trichlorophenoxyacetic acid (2,4,5-T). Cytokinins included kinetin, N-(2-chloro-4-pyridyl)-N′- pheny- lurea (CPPU), thidiazuron (TDZ), and zeatin. Woody plant medium (WPM) salts containing 3% sucrose and 0.7 % agar were used as the basic culture medium. Differences in the number of shoots (primodia), morphology of micropagated shoots, and amount of callus were observed between the plant growth regulator treatments and concentrations. Explants cultured in media containing cytokinins produced more shoots (primodia) with TDZ producing the most, followed by CPPU, kinetin and zeatin. Explants cultured in media containing auxins enhanced callus induction with 2,4,5-T achieving the most, followed by 2,4-D, IBA and NAA.关键词
板栗/生长素/细胞机动素/组织培养Key words
chestnut/auxin/cytokinin/tissue culture分类
农业科技引用本文复制引用
杨国臣,鲁重格,Theophilus M. Asante,Paul E.Read..美洲板栗愈伤组织诱导和植株再生的研究[J].吉林农业大学学报,2008,30(4):466-471,6.基金项目
美国农业部(USDA/CSREES,NCX-169-5-01-130) （USDA/CSREES,NCX-169-5-01-130）
