中德临床肿瘤学杂志（英文版）2009，Vol.8Issue(8)：467-470,4.DOI:10.1007/s10330-009-0091-x
白藜芦醇对食管癌细胞凋亡相关基因survivin和bax表达的影响
Impact of resveratrol on the expression of apoptosis related gene survivin and bax in human cancer cells
摘要
Abstract
Objective: We explored the mechanism of apoptosis in human esophageal cancer Eca109 cells by resveratrol.Methods: The suppressive ratio of resveratrol on Ecal09 cells proliferation was evaluated by MTT colorimetric assay and morphology was observed by transmission electron microscope. The expression of survivin and bax was analyzed by RT-PCR and Flow Cytometry (FCM). Results: Resveratrel inhibited the growth of Ecal09 cells in a dose-and time-dependent man-ner, and the suppressive ratio arrived at 76.42%. Morphological apoptosis could be observed after treated with resveratrol.The bulk of some drug-treated cells turned small and the nuclear chromatin became condensed and marginated. The results determined by RT-PCR and FCM showed that resveratrol could down-regulate surviving, while up-regulate bax. Conclusion:Resveratrol could induce the apoptosis of human esophageal cancer Eca109 cells, and its possible molecular mechanisms might be related to modulation the expression of survivin and bax.关键词
resveratrol/esophageal neoplasms/Ecal09 cell/cell apoptosis/survivin/baxKey words
resveratrol/esophageal neoplasms/Ecal09 cell/cell apoptosis/survivin/bax分类
医药卫生引用本文复制引用
Yongjun Li,Xiaohui Sun,Rui Zhang..白藜芦醇对食管癌细胞凋亡相关基因survivin和bax表达的影响[J].中德临床肿瘤学杂志（英文版）,2009,8(8):467-470,4.基金项目
a grant from the Science Research and DevelopmentProgram of Hepei Province (No. 062761835). （No. 062761835）
