南京大学学报（自然科学版）2006，Vol.42Issue(5)：470-478,9.
鱼藤酮诱导SH-SY5Y细胞对过氧化氢损伤易感性增高与硫氧还蛋白下调有关
Increased Susceptibility to H2O2 in SH-SY5Y Cells Affected by Rotenone Is Accompanied by the Decrease of Thioredoxin
丁镇 1丁红群 1谢丽 1赵晓宁 1杨晓荷 1高静1
作者信息
摘要
Abstract
The inhibition of mitochondrial respiratory chain complex Ⅰ and oxidative damage have been implicated in the pathogenesis of Parkinson's disease (PD). To study the alteration of cellular antioxidant capability with complex Ⅰ impairment, human neuroblastoma SH-SY5Y cells were treated by specific mitochondrial complex Ⅰ inhibitor rotenone and followed by H2O2 exposure. The susceptibility of complex I inhibition cells to H2O2 was found to be increased in a dose-dependent manner in cell morphology observation and cell viability detection. Meanwhile, one of antioxidant proteins, thioredoxin, was downregulated following the oxidative stress, which suggests the potential role of thioredoxin as a neuronal injury index as well as a therapeutic target for neuroprotection.关键词
线粒化/氧化应激/鱼藤酮/硫氧还蛋白/帕金森病Key words
mitochondria/oxidative stress/rotenone/thioredoxin/Parkinson's disease分类
医药卫生引用本文复制引用
丁镇,丁红群,谢丽,赵晓宁,杨晓荷,高静..鱼藤酮诱导SH-SY5Y细胞对过氧化氢损伤易感性增高与硫氧还蛋白下调有关[J].南京大学学报（自然科学版）,2006,42(5):470-478,9.
