中国农业科学（英文版）2006，Vol.5Issue(6)：473-477,5.
The Effect of Tryptophan on Serotonin-Like Neurons in Duck Cerebellum
The Effect of Tryptophan on Serotonin-Like Neurons in Duck Cerebellum
摘要
Abstract
Healthy Cherry Valley ducks were used in the present study. Different doses of tryptophan were injected intraperitoneallyto them after being fasted 4 h (8:00 a.m.-12:00 a.m.). One hour later, they were deeply anaesthetized and perfused. The cerebellum was removed to make serial paraffin longitudinal sections. The streptavidin-biotin-peroxidase complex (SABC) method was used to study the distribution of serotonin-like neurons in the cerebellum. All films were analysed by using a computer-assisted image analysis system. Serotonin-like neurons are only localized in cerebellar Purkinje cell layer. The optical density averages of serotonin-like neurons in 200 and 100 mg kg-1 group are significantly higher than that of O mg kg-1 group (P＜0.01). These results show that serotonin-like neurons are distributed in Purkinje cell layer and that excessive tryptophan can affect the content of serotonin in cerebellum.关键词
tryptophan/serotonin/cerebellum/Cherry Valley duck/streptavidin-biotin-peroxidase complex (SABC)Key words
tryptophan/serotonin/cerebellum/Cherry Valley duck/streptavidin-biotin-peroxidase complex (SABC)分类
农业科技引用本文复制引用
LIU Hua-zhen,TANG Wen-hua,PENG Ke-mei,CHEN Wen-qin,LUO Guan-zhong,WANG Yan,WEI Lan..The Effect of Tryptophan on Serotonin-Like Neurons in Duck Cerebellum[J].中国农业科学（英文版）,2006,5(6):473-477,5.基金项目
This study was supported by the National Natural Science Foundation of China (39970547) and Natural Science Fund of Hubei Province (98J012). （39970547）
