环境与职业医学2006，Vol.23Issue(6)：474-478,5.
三羟基异黄酮对日本比目鱼(牙鲆)性腺分化的影响
Effects of Genistein on Gonadal Sex Differentiation in Japanese Flounder (Paralichthys Olivaceus)
杨隽 1郭红卫 2仲伟鉴 1张胜年 2安达龙太 2北野健3
作者信息
- 1. 复旦大学公共卫生学院,上海,200032
- 2. 上海市疾病预防控制中心,上海,200336
- 3. 熊本大学自然科学研究科,日本,熊本市,860-8555
- 折叠
摘要
Abstract
[ Objective ] To investigate the estrogenic effects of genistein(GEN) on the gonadal sex differentiation in Japanese flounder, a teleost that exhibits temperature-dependent sex determination. [ Methods ] Treatment with GEN was carried out by providing the flounder larvae with an artificial diet mixed with 10 μg/g and 100μg/g diet of GEN at a masculinizing temperature (27 ℃ ) from 30th to 100th day after hatching(dah). Phenotypic sex of the fish was determined at 100th (juvenile stage)inhibiting substance) mRNAs expression in the genetically female flounder treated with GEN were examined by in situ hybridization and RT-PCR analyses. [ Results ] The results showed that treatment with 10 μg/g and 100 tg/g diet of GEN dose- dependently induced feminization of the flounder larvae reared at 27 ℃ with female percentages of 46.7% and 96.7%, respectively. The result of in situ hybridization and RT-PCR analyses showed that feminization of the larvae by GEN was accompanied by up-regulation of P450arom mRNA expression and down-regulation of MIS mRNA expression in female gonad at 100th day after hatching.[ Conclusion ] GEN has estrogenic action on gonadal sex differentiation in Japanese flounder and its mechanism may be due to the induction of P450arom mRNA expression and the suppression of MIS mRNA expression.关键词
日本比目鱼/性分化/三羟基异黄酮/P450芳香化酶/苗勒氏管抑制物质Key words
Japanese flounder/sex differentiation/genistein/P450 aromatase/Müllerian inhibiting substance分类
医药卫生引用本文复制引用
杨隽,郭红卫,仲伟鉴,张胜年,安达龙太,北野健..三羟基异黄酮对日本比目鱼(牙鲆)性腺分化的影响[J].环境与职业医学,2006,23(6):474-478,5.
