首页|期刊导航|中国农业科学（英文版）|Comparative Study on the Infectivity and Spore Surface Protein of Nosema bombycis and Its Morphological Variant Strain
中国农业科学（英文版）2005，Vol.4Issue(6)：475-480,6.
Comparative Study on the Infectivity and Spore Surface Protein of Nosema bombycis and Its Morphological Variant Strain
Comparative Study on the Infectivity and Spore Surface Protein of Nosema bombycis and Its Morphological Variant Strain
摘要
Abstract
A new morphological variant strain of microsporidium was produced by infecting the mulberry looper, Hemerophila atrilineata [Phthonandria atrilineata], with Nosema bombycis successively for 24 times, and named 24Nbh. Comparative studies on morphology, infectivity and spore surface protein were conducted. 24Nbh was short and wide, and had a significant difference (P＜0.01) over the Nb spores. The infectivity tests conducted on second instar silkworm larvae showed that IC50 of 24Nbh was 1.98× 104 spores mL-1 and of Nb was 1.72× 103 spores mL-1, thus indicating that the infectivity of Nb decreased 11.5 times after multiplying in mulberry looper for 24 times. The IC50 of spores from silkworm infected with 24 Nbh was 6.9 times less than Nb, showing that the infectivity of 24Nbh spores rejuvenated very fast when reinfected to silkworms, further more, the length and width of such spore was larger than 24Nbh (P＜0.01) and smaller than Nb (P＜0.05).The SDS-PAGE profiles of Nb and 24Nbh were generally the same, 4 distinct proteins of 12, 17, 30, 33 kDa were obtained with difference in quantity. When 120 μg of protein was applied for 2D-PAGE, five suspected different proteins with difference in quantity were observed. These results demonstrate that these differential proteins maybe associated with variation in infectivity of the spores.关键词
Nosema bombycis/Morphological variation/Infectivity/Surface protein/Two dimensional electrophoresisKey words
Nosema bombycis/Morphological variation/Infectivity/Surface protein/Two dimensional electrophoresis分类
农业科技引用本文复制引用
HUANG Shao-kang,LU Xing-meng..Comparative Study on the Infectivity and Spore Surface Protein of Nosema bombycis and Its Morphological Variant Strain[J].中国农业科学（英文版）,2005,4(6):475-480,6.基金项目
This study was funded by the National Natural Science Foundation of China(30270898). We are grateful to Dr.Shyam Kumar for manuscript correction. （30270898）
