中国组织工程研究与临床康复2009，Vol.13Issue(24)：4791-4795,5.
水通道蛋白8在人胎盘胎膜中的表达
Aquaporin 8 expression in human placenta and fetal membrane
摘要
Abstract
BACKGROUND: Placenta and fetal membrane play an important role In maternal-fetal homeostasis. However, the molecular and cellular mechanisms underlying water transfer across placenta and amniotic membrane remain unknown. It is hypothesized that maternal-fetal fluid exchanges via aquaporin (AQP) water channels in the placenta and fetal membrane.OBJECTIVE: To investigate AQP8 protein expression in normal human placenta and fetal membrane.DESIGN, TIME AND SETTING: A control observation was performed at the Central Laboratory of Guangzhou Medical College from July to December 2005.MATERIALS: Human placenta and fetal membrane tissues from 5 elective cesarean section deliveries of normal term pregnancies (range 37-42 weeks) were studied. Maternal age averaged (27?) years old. Experimental protocol was approved by the Hospital's Ethics Committee.METHODS: Thirty minutes after delivery, fetal membrane and placenta were dissected and washed with sterile physiological saline. Some were frozen at -80?, and the remaining tissues were fixed for 24-48 hours with 10% neutral formalin and paraffin embedded for immunohistochemical staining.MAIN OUTCOME MEASURES: AQP8 expression and distribution in human placenta and fetal membrane were detected by the reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blotting analysis.RESULTS: RT-PCR results showed that AQP8 mRNA was expressed in both placenta and fetal membrane tissues. Western blotting analysis also yielded positive results in placenta and fetal membrane with a specific band site at approximately 45 000.Immunohistochemistry results revealed that AQP8 protein was expressed in placental syncytiotrophoblasts, amniotic epithelial cells, and chorion cytotrophoblasts.CONCLUSION: At protein level, AQP8 is expressed in placental syncytiotrophoblasts, amniotic epithelial cells, and chorion cytotrophoblasts.关键词
水通道蛋白8/基因表达/胎盘/胎膜/母胎液体平衡分类
医药卫生引用本文复制引用
刘慧妹,郝荣增,宋小飞,熊正方..水通道蛋白8在人胎盘胎膜中的表达[J].中国组织工程研究与临床康复,2009,13(24):4791-4795,5.基金项目
国家自然科学基金资助(30471828) （30471828）
教育部留学回国人员科研启动基金(教外司留[2005]383号) （教外司留[2005]383号）
广东省科技计划项目基金资助(2004B36001008) （2004B36001008）
广州市卫生局科研基金(28)the National Natural Science Foundation of China, No.30471828 （28）
Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, No. 2005-383 （）
Science and Technology Planning Project of Guangdong Province, No. 2004B36001008 （）
Scientific Research Foundation of Health Administration of Guangzhou City, No.28 （）
