作物学报2000，Vol.26Issue(4)：482-489,8.
水稻体细胞无性系雄性不育突变
Male Sterile Mutants from Rice Somaclones
范树国 1梁承邺 1刘鸿先1
作者信息
- 1. 中国科学院华南植物研究所,广东,广州,510650
- 折叠
摘要
Abstract
Young panicles from fiva rice varieties，Zhenshan 97A(WA type cytoplasmic malesterile(CMS)line，indica)，Hongyuan A(HL type CMS line，indica)，Baoyuan A(BT type CMS line，indica)，Nongken 58s(photoperiod-sensitive genic male-sterile rice(PGMR)，japonica)and W6154s(thermo-sensitive genic male-sterile rice(TGMR)，indica)，were cultured on the other was coded S14-1, TAT) and Baoyuan A (one was coded 28-5, SpAT; the other wascoded 28-19, TAT), kept sterile continuously for five generations when they were backcrossedwith the ten parents stated above.The other five male-sterile variants, which derived from Hongyuan A (coded 24-5),Baoyuan A(coded 28-35) and W6154s(coded WP-l, WP-2 and WS) turned into fertility with alot of fertile pollens and very high self seed set rate (more than 80%) when they were crossed with the ten parents stated above.different media． After obtained regenerating plants，examined pollen fertility，madetestcrosses of male-sterile variants，did genetic analyses of male-sterile variants，discussedrestoring and maintaining relationship of male sterile variants，we found 10 cases of male-sterilevariants in three rice variedes，Hongyuan A，Baoyuan A and W6154s．Abortive pollen of ricecould be classified into four types：pollen free type(PFT)，typical abortion type (TAT)，pherical abortion type(SpAT)and stained abortion type(StAT)．The phenomenon that type of polln abortion could change from one to another through tissue culture was found in ricesomaclones in R1 generaton．Male-sterile variants in R1 generation were crossed with ten typical maintainer lines and restorer lines(Zhenshan 97B，Erjiuai 4B，Hongyuan B，Baoyuan B，Taiyin l，Minghui 63，Ce64，IR24，IR26 and IR36)to investigate the restoring and maintaining relationship of thesemale-sterile variants．The main results were as follows：The restoring and maintaining relationship of one typical abortion type(TAT)variant in Rlregenerated plants which derived from Baoyuan A(coded 28-18)was the same as that of WAtype．The variant had been backcrossed with their maintainer lines for five generations，and keptsterile all along．Four male—sterile variants，which derived from Hongyuan A(one was coded 24-1，StAT；关键词
水稻/体细胞无性系/幼穗培养/核质互作雄性不育突变体/恢保关系Key words
Oryza sativa L./Somaclone/Young panicle culture/CMS mutants/Restoring and maintaining relationship分类
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范树国,梁承邺,刘鸿先..水稻体细胞无性系雄性不育突变[J].作物学报,2000,26(4):482-489,8.
