华中师范大学学报（自然科学版）2001，Vol.35Issue(1)：48-50,3.
二苯胺-H2O2-HRP伏安酶联免疫分析新体系的研究
Determination of HRP with coupling reactionvoltammetric enzyme-linked immunoassay based on diphenylamine-H2O2-HRP system
摘要
Abstract
HRP has been determined by coupling reaction of voltammetricenzyme-linked immunoassay based on diphenylamine-H2O2-HRP system for the first time. HRP catalyes the reaction of H2O2 oxidizing diphenylamine,the product of which has sensitive second order derivative linear-sweep voltammetric wave at the potential of -0.53 V(vs.SCE) in pH=8.5 Britton-Robinson buffer solution. Trace amount of HRP may be determined by the detection limit of 1.0×10-9 g/mL HRP and the linear range is 4.0×10-9～2.0×10-7 g/mL.关键词
辣根过氧化物酶（HRP）/二苯胺/伏安酶联免疫分析法分类
化学化工引用本文复制引用
王军,张锡贞,孙伟,焦奎..二苯胺-H2O2-HRP伏安酶联免疫分析新体系的研究[J].华中师范大学学报（自然科学版）,2001,35(1):48-50,3.基金项目
国家自然科学基金资助项目 （）
