中国临床康复2004，Vol.8Issue(23)：4864-4865,2.
碱性成纤维细胞生长因子与重组人骨形态发生蛋白-2诱导成骨能力的量效关系
Dose-dependent stimulation of osteoinduction by recombinant human bone morphogenetic protein-2 enhanced by basic fibroblast growth factor
摘要
Abstract
AIM: To study the dose-dependent stimulation of osteoinduction by recombinant human bone morphogenetic protein-2(rhBMP-2) enhanced by basic fibroblast growth factor(bFGF).METHODS: Totally 280 Balb/c mice were divided into 8 groups randomly by throwing coin, with 35 mice in each group. The dosage of rhBMP-2 was 0. 5 mg and the dosage of bFGF was 0, 2, 10, 40, 80, 300, 500 active unit (IU) respectively; and the simple calf spongy bone carrier was treated as the control group. According to the experiment design, rhBMP-2 suspension,bFGF-PVP solution and carriers were mixed completely before vacuum suction and freeze-drying. Compound bone grafting materials were implanted into the right thigh pouches of the mice and samples were extracted respectively 3, 5, 7, 9, 14, 21 days after the implantation. Then histological and X-ray analysis were taken and calcium content was measured in order to observe the osteoinduction and osteogenesis of each group.RESULTS: In 10 - 80 IU bFGF groups, the proliferation and differentiation of mesenchymal cells, formation of chondrocytes and new bones were all earlier than those in other groups; The amount of osteogenesis was greater than that in other groups; and the calcium content was obviously higher than that in other groups( t =4.44, P ＜ 0.05) . These indicated that bFGF of these dosage could obviously enhance the osteoinduction and osteogenesis by rhBMP-2. In 0, 2, 300 IU bFGF groups, there was no significant difference about the proliferation and differentiation of mesenchymal cells, formation of chondrocytes and new bones in each group ( t = 0.73, P ＞ 0. 05) . These suggested that bFGF of these dosage had no obvious influence on the osteoinduction and osteogenesis by rhBMP-2. In 500 IU bFGF group, only few scattered new bones were seen in just one sample 12 days after the implantation and the calcium content was obviously lower than that in other groups( t = 6. 15, P ＜0. 05) . These suggested that bFGF of this dosage could obviously inhibit the osteoinduction and osteogenesis by rhBMP-2. Twelve days after the implantation, no new bones were seen in simple spongy bone carrier group.CONCLUSION: The dose-dependent stimulation of osteoinduction by rhBMP-2 enhanced by bFGF does exist.关键词
骨形态发生蛋白质类/成纤维细胞生长因子,碱性/骨生成分类
医药卫生引用本文复制引用
杜俊杰,罗卓荆,胡蕴玉,李明全,曹艳杰,袁志,吕荣..碱性成纤维细胞生长因子与重组人骨形态发生蛋白-2诱导成骨能力的量效关系[J].中国临床康复,2004,8(23):4864-4865,2.基金项目
国家自然科学基金资助项目(39600148) （39600148）
