中国临床康复2004，Vol.8Issue(23)：4888-4889,2.
特发性脊柱侧凸椎旁肌组织Bcl-2蛋白表达及细胞凋亡的研究
Apoptosis and expression of Bcl-2 in the paraspinal muscles of idiopathic scoliosis
赵宇 1邱贵兴1
作者信息
- 1. 中国医学科学院中国协和医科大学北京协和医院骨科,北京市,100730
- 折叠
摘要
Abstract
BACKGROUND: The etiology of idiopathic scoliosis is still uncertain. The paraspinal muscles have been implicated by several investigators as a possible causative factor in the production and progression of adolescent idiopathic scoliosis. Therefore, the role of the spinal musculature in the pathogenesis of scoliosis has been the subject of much investigation.OBJECTIVE: This study focused on the expressive difference among Bcl-2,Caspase-3 and bcl-x of the thoracic spinal musculature on convex side with those on the concave side in the scoliosis patients in order to explore the possible mechanism which paraspinal muscles play on scoliosis from the view of molecular biology.DESIGN:A randomized case-control study was conducted.SETTING and PARTICIPANTS: This research was completed in Department of Orthopaedics of Peking Union Hospital. Two patients with bursting fracture of thoracic vertebra and lumbar were selected as control group. The research group was composed by 10 patients which including 2 males and 8 females with scoliosis of thoracic vertebra, aged from 12 to 17 years old,mean age was 14. 3. The average Cobb angel was 57.7°(ranged from 45°～85°).INTERVENTION: Paraspinal muscles were taken from both sides during surgery from the apex of the curve between the 6th and 11th thoracic vertebral levels. Part of the tissue was fixed in formalin and stained with hematoxylin and eosin; the remaining tissue was snap frozen and processed for immunohistochemistry and Western blotting.muscles.RESULTS: The expression of Bcl-2 in convex side of paraspinal muscles was reduced. There was no difference between scoliosis patients and control group on cell apoptosis because it could be seen in both groups. Compared with concave side of scoliosis and control group, the muscle fibers were much thinner in convex side.CONCLUSION: The asymmetry of paraspinal muscles caused by anomaly of nerve and muscles may be the important factor which leads to the development of idiopathic scoliosis. This asymmetry is probably correlated with the asymmetric expression of Bcl-2.关键词
脊柱侧凸/脊椎/肌疾病/病因学分类
医药卫生引用本文复制引用
赵宇,邱贵兴..特发性脊柱侧凸椎旁肌组织Bcl-2蛋白表达及细胞凋亡的研究[J].中国临床康复,2004,8(23):4888-4889,2.
