作物学报2000，Vol.26Issue(4)：490-495,6.
水稻原生质体愈伤组织再生植株培养程序的比较
Comparison of Culture Procedures for Regeneration of Plants from Protoplast-derived Calluses of Rice(Oryza sativa L. )
杨跃生 1简玉瑜 1陈远玲1
作者信息
- 1. 华南农业大学生物技术学院,广东,广州,510642
- 折叠
摘要
Abstract
Four different culture procedures combined with several treating methods werecompared for their effects on regeneration of plants from protoplast-derived calluses of rice. Only a small number of weak plants could be regenerated when the calluses were transferred and cultured directly from a proliferation medium containing 2, 4-D to a plant regeneration medium containing BA and NAA (procedure 1). Addition of ABA to the proliferation medium induced the formation of nodular calluses with enhanced regeneration potential (procedure 2 ), whileaddition of ABA to the regeneration medium resulted in the formation of compact calluses withsuppressed adventitious buds, which would grow fast upon transfer to a growth medium free of ABA (procedure 3 ). By Culturing of the calluses consequently on ABA supplementedproliferation medium, ABA supplemented regeneration medium and the growth medium(procedure 4), large number of more healthy plants was obtained. Statistical test indicates thatprocedure 2 and 3 were mumore efficient than procedure l, while procedure 4 was the mostefficient for plant regeneration.关键词
植株再生/原生质体/愈伤组织/脱落酸/水稻Key words
Plant regeneration/Protoplast/Callus/Abscisic acid/Rice分类
农业科技引用本文复制引用
杨跃生,简玉瑜,陈远玲..水稻原生质体愈伤组织再生植株培养程序的比较[J].作物学报,2000,26(4):490-495,6.
