中国临床康复2004，Vol.8Issue(23)：4913-4915,3.
荷瘤裸鼠放射自显影受体辨别的实验研究
Receptor discrimination with autoradiography in tumor-bearing nude mice
李前伟 1郑秀海 2张广运 1王曙光2
作者信息
- 1. 解放军第三军医大学西南医院,核医学中心,重庆市,400038
- 2. 解放军第三军医大学西南医院,全军肝胆外科研究所,重庆市,400038
- 折叠
摘要
Abstract
BACKGROUND: Tumor receptor scintigraphy by using small molecule peptide labeled with radionucide is nowadays one of the foremost border research domain in molecular nuclear medicine. To aim directly at the high density of alpha(v) beta(3) receptor expressing in tumor tissues, it is the research hotspot of tumor receptor imaging at present to develop high selectivity αvβ3 receptor antagonist containing RGD motif labeled with radionucide.OBJECTIVE: To investigate the possibility of RGD-4CY labeled with radionuclide to perform tumor αvβ3 receptor scintigraphy according to the basis of experimental animal.DESIGN: A completely randomized controlled trial.SETTING and PARTICIPANTS: The experiment was completed in Nuclear Medicine Center, Southwest Hospital, Third Military Medical University of Chinese PLA. Twenty-five healthy mice and fifteen QBC939(human cholangiocarcinoma) with a mass of( 15 -20) g and aged 4 -6 weeks were provided by the center of Third Military Medical University of Chinese PLA.INTERVENTION: RGD-4CY was labeled with 125I by using routine Iogogen method, subsequently separated by SEP-PAK C18 column. The biodistribution of 125I-RGD-4CY was studied separately in 25 normal mice and 15 nude mice bearing human QBC939 cholangiocarcinoma after venous injection of 125I-RGD-4CY 0. 1 mL(370 kBq) at 10, 30, 60, 120 and 240 minutes, and the autoradiography was performed in 3 tumor-bearing nude mice after venous injection of vein at 1 hour.MAIN OUTCOME MEASURES: The radiochemical purity of 125I-RGD-4Cy;the biodistribution and kinetic characteristics of 125I-RGD-4CY; the radioactivity ratio of tumor tissues(T) and no-tumor tissues(NT) and the radioactivity distribution of tumor and normal tissues on the autoradiography images of tumor-bearing nude mice were mesured.RESULTS: The radiochemical purity of 125I-RGD-4CY was 99%. Biodistribution showed that the radioactive descent was rapid in blood and liver; The radioactivity was mainly eliminated through kidneys; The amount of radioactivity was only 32% and 44% respectively in lungs and muscles of the liver at 120 minutes( t values were 3.33 and 3.64, P ＜ 0. 05). The tumor radioactivity was higher than that of lungs, muscles and intestines at all phases in tumor-bearing nude mice( t values were 3.18 - 13.24, P ＜ 0. 05), and the T/NT radioactive ratio was 26.0, 26.0 and 8.7 respectively at 240 minutes.Autoradiography displayed that the radioactive image was the highest in tumor region and there was only a small quantity radioactive distribution in lungs,muscles, neck and other soft tissues.CONCLUSION: RGD-4CY labeled with γ-emitter nuclide is a potential tracer of tumor αvβ3 receptor scintigraphy.关键词
肿瘤,实验性/小鼠,裸/放射自显影术分类
医药卫生引用本文复制引用
李前伟,郑秀海,张广运,王曙光..荷瘤裸鼠放射自显影受体辨别的实验研究[J].中国临床康复,2004,8(23):4913-4915,3.
