中国临床康复2004，Vol.8Issue(23)：4930-4931,2.
雷公藤多甙对同种异体神经移植免疫抑制作用的实验研究
Experimental study of the immuno-suppressive effect of tripterygium on nerve allograft
摘要
Abstract
BACKGROUND: Cyclosprone A( CSA ), an immuno-suppressive agent used in peripheral nerve allograft, is of high price. However, tripterygium,a traditional Chinese medicine, was previously reported to have the function of immuno-suppression.OBJECTIVE: To verify the possibility of using tripterygium as a substitution of CSA in nerve allografts.DESIGN: Double blinded and randomized design.SETTING and PARTICIPANTS: Adult healthy male SD(48) and Wistar rats(18) of SPF grade, weighing 180 -210 g,were provided by the Medical Animal experimental Center of Wuhan University.INTERVENTIONS: Forty-eight SD rats as recipients of sciatic nerve allograft were randomly assigned into one of the following groups: Autograft Group; CSA Group, allograft with CSA 5mg/kg per day for 5 weeks;Tripterygium Group, allograft with tripterygium 5mg/kg per day for 5 weeks; and Control Group, allograft withnot immune treatment. Eighteen Wistar rats were the donors of the sciatic nerve allograft.MAIN OUTCOME MEASURES: Observation was done under light microscope and electron microscope examination 12 weeks post-operatively.The number of axons in the middle segment of the allogeneic graft was counted, double sides of gastrocnemius muscle were weighed and electrophysiological parameters were also measured.RESULTS: At 12 weeks after transplantation, latent period peak of tripterygium Groups , evoked potential nerve conduction velocity , accumulated points of amplitude, the wet mass of muscle, the recovery degree of muscle's wet mass and the number of axis-cylinder recovered of the autograft, CSA and tripterygium group were better than those of their control counterpart(q=3.95-8.32, P ＜ 0.01) .There were good regenerate nervus form and functional recovery.CONCLUSIONS: Tripterygium as an immuno-suppressant has the similar effect to CSA on nerve allograft.关键词
周围神经/移植,同种/免疫抑制剂/环孢菌素/雷公藤分类
医药卫生引用本文复制引用
李皓桓,刘世清,彭昊,王志林..雷公藤多甙对同种异体神经移植免疫抑制作用的实验研究[J].中国临床康复,2004,8(23):4930-4931,2.基金项目
湖北省科委重点攻关课题(982P1514) （982P1514）
