解放军医学杂志2001,Vol.26Issue(1):49-51,3.
PSP94抗血清制备及正常、增生和癌变前列腺人外周血PSP94分析
PREPARATION OF ANTI-PSP94 SERUM AND ANALYSIS OF HUMAN PERIPHERAL BLOOD FROM NORMAL PERSONS AND PATIENTS WITH BENIGN PROSTATE HYPERPLASIA AND PROSTATE CARCINOMA
刘建香 1刘丽 1刘立忠 1苏旭 2刘树铮 2冷爱军 1曹颖1
作者信息
- 1. 空军总医院
- 2. 白求恩医科大学预防医学院放射生物教研室
- 折叠
摘要
Abstract
Recombinant plasmid pGEX-PSP94 expressing fusion protein GST-PSP94 was constructed and expressed fusion protein GST-PSP94 in E.coli.Anti-PSP94 serum was obtained from immunized rabbits using GST-PSP94 protein after purified by affinity chromatography.Immunology analysis indicated that anti-PSP94 serum can specifically bind to PSP94 recombinant protein.Analysis of PSP94 concentration in human peripheral blood from ten normal persons,ten patients with benign prostate hyperplasia(BPH) and five cases with prostate carcinoma showed that no significant difference of PSP94 concentration is found in the serum from control and experimental groups.The results suggested that PSP94 can not be regarded as a serum marker of prostate carcinoma.关键词
前列腺增生/前列腺肿瘤/基因表达分类
生物科学引用本文复制引用
刘建香,刘丽,刘立忠,苏旭,刘树铮,冷爱军,曹颖..PSP94抗血清制备及正常、增生和癌变前列腺人外周血PSP94分析[J].解放军医学杂志,2001,26(1):49-51,3.