眼科学报2003，Vol.19Issue(1)：49-53,5.
Adeno-associated Virus Mediated LacZ Gene Transfect to Cultured Human Iris Pigment Epithelium Cells
Adeno-associated Virus Mediated LacZ Gene Transfect to Cultured Human Iris Pigment Epithelium Cells
Chun Zhang 1Shibo Tang 1Yan Luo 1Xiaoling Liang 1Jing Ma 1Shaofen Lin1
作者信息
- 1. Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China
- 折叠
摘要
Abstract
Purpose: To study the feasibility of adeno-associated virus mediated gene transfection tocultured human iris pigment epithelium (IPE) cells in vitro.Methods: Recombinant replication deficient adeno-associated viruses (AAV) expressingLacZ gene were produced without helper virus. The LacZ gene was transduced into culturedhuman IPE cells.Results: Cultured human IPE cells stained positively anticytokeratin, The titer ofrAAV-LacZ was 2.1 × 108 virus particles/ml, 42% cultured human IPE cells expressedβ-galactosidase 7 days after transfection and 67% after 14 days.Conclusions: Recombined AAV produced without helper virus can transfer a foreign geneinto human IPE cells with high efficiency in vitro.关键词
adeno-associated virus/iris pigment epithelium cellKey words
adeno-associated virus/iris pigment epithelium cell分类
医药卫生引用本文复制引用
Chun Zhang,Shibo Tang,Yan Luo,Xiaoling Liang,Jing Ma,Shaofen Lin..Adeno-associated Virus Mediated LacZ Gene Transfect to Cultured Human Iris Pigment Epithelium Cells[J].眼科学报,2003,19(1):49-53,5.
