癌症2001，Vol.20Issue(5)：496-498,3.
转染 p53基因对肺腺癌细胞株裸鼠 移植瘤生长的影响
Study on the Role of p53 Gene Transfer on Human
Glandular Lung Cancer Cell Growth in Nude Mice
李萍 1王北宁 1丁振若2
作者信息
- 1. 北京军区总医院检验科,
- 2. 第四军医大学西京医院临床分子生物学研究室,
- 折叠
摘要
Abstract
Objective： This study was designed to explore the significance and the role of wild-type p53 (wt-p53) gene and mutant p53 gene(mt-p53) transfer on human glandular lung cancer cell growth in nude mice. Methods： wt-p53 gene and mt-p53 gene were transfected and lipofectin-mediated into the human glandular lung cancer cell line GLC-82. And the growth of gene-transfected cell lines were observed in vitro and in vivo. Results： The colony number in the colong-forming experiment and the volume and weight in nude mice were greater in the mf-p53 tranfecting cells group than in the control group. The tumor resulting from the cells transfected with the wt-p53 gene grew more slowly and was smaller than that from control GLC-82 cells. In contrast, the tumor from the cells transfected with the mt-p53 gene grew faster than that produced by cells transfeted with the wt-p53 gene and that produced by control GLC-82 cells. Conclusion： The wild-type p53 gene could inhibit the glandular lung cancer cell growth in nude mice and the mutant p53 gene could enhance the glandular lung cancer cell growth in nude mice.关键词
抑癌基因/p53基因/肺腺癌/裸鼠致瘤性分类
医药卫生引用本文复制引用
李萍,王北宁,丁振若..转染 p53基因对肺腺癌细胞株裸鼠 移植瘤生长的影响[J].癌症,2001,20(5):496-498,3.
