中国组织工程研究与临床康复2009，Vol.13Issue(25)：4981-4984,4.DOI:10.3969/j.issn.1673-8225.2009.25.046
镍钛形状记忆合金表面改性后与成骨细胞的生物相容性
Biocompatibility of surface modified Nickel-Titanium shape memory alloy with osteoblasts cultured in vitro
李强 1辛林伟 1夏亚一 2张钦 2刘文忠 2吴萌2
作者信息
- 1. 桂林医学院附属医院骨科,广西壮族自治区桂林市541001
- 2. 兰州大学第二医院骨科研究所,甘肃省兰州市730030
- 折叠
摘要
Abstract
BACKGROUND: Although shape memory alloy has been extensively used in modem medicine, including orthopaedics and dental surgery, the body fluid could influence the stability of biomaterial and some ions released by materials may cause toxic and side effect to human body. The technology for the modification of shape-memory materials is of crucial importance for clinical use of shape memory alloy.OBJECTIVE: To investigate the biocompatibility of diamond-like carbon (DLC) coated Nickel-Titanium shape memory alloy and uncoated shape memory alloy with osteoblasts cultured in vitro. DESIGN, TIME AND SETTING: A comparative observation. The study was performed at the Institute of Othopaedics of Lanzhou University from March to June 2005.MATERIALS: A total of 30 DLC-coated Nickel-Titanium shape memory alloy disks, 6 minx7 mmx0.5 mm, and the same amount of uncoated ones of equal size were provided by College of Materials, Lanzhou University. METHODS: Rabbit osteoblast suspension of the third passage at density of 10 × 108/L were incubated with DLC-coated Nickel-Titanium shape memory alloy disks and uncoated shape memory alloy in 12-well culture plate in 5% CO2 at 37 ℃.MAIN OUTCOME MEASURES: Culture media were counted at 1, 2, 3, 4 and 5 days of culture to determine alkaline phosphatase (AKP) activity and nickel (Ni2+) concentration.RESULTS: The proliferation of osteoblasts and the concentration of AKP in DLC- coated group were greater than uncoated group; while the uncoated group released more Ni2. into the cells culture media than coated group (P < 0.05). CONCLUSION: DLC-ceated Nickel-Titanium shape memory alloy appears to have better biocompatibility with osteoblast cultured in vitro compared to uncoated ones.关键词
类金刚石/镍钛记忆合金/表面改性/成骨细胞/生物相容性分类
医药卫生引用本文复制引用
李强,辛林伟,夏亚一,张钦,刘文忠,吴萌..镍钛形状记忆合金表面改性后与成骨细胞的生物相容性[J].中国组织工程研究与临床康复,2009,13(25):4981-4984,4.
